The conidiophores of Penicillium are hyaline, smooth and swollen at the top. They have one or more whorls on which 10 to 16 phyalids are attached (Figures 1 and 2). These bottle-shaped phyalids can be produced alone by the conidiophore or from metules (Figure 3).
The conidia are formed from the phyalides on top of which they are assembled into compact long columns. Each unicellular conidium measures 3-3.5 µm in diameter. They are smooth and globular, most often dull green. Very easily transportable by the wind, the conidia are released in the form of a cloud of green dust.
The penicillus , a characteristic structure of Penicillium spp., Is made up of all conidiophores, metules, phyalids and conidia.
Several types of ramifications are possible in Penicillium spp .. Some are monoverticillate, or unbranched, others are biverticillate, which corresponds to the appearance of a branching level, or even triverticillate (2 branching levels) . This character contributes to the discrimination between species. It is also at the origin of the "brush" or "brush" appearance of the fruiting of Penicillium bodies observed with a binocular magnifying glass or with a microspcope. For example, P. expansum is triverticillate (figure 3) while P. frequentans is monoverticillate.
Appearance of the colonies on malt-agar medium : they grow rapidly on malt-agar medium and exhibit a powdery appearance corresponding to the dense felting of conidiophores. In general, cultures Penicillium are stained in shades of green (Figure 4). P. expansum forms a dark green colony with an orange to light brown underside. A very characteristic rotten apple smell emanates from it.