Final diagnosis


Cercospora nicotianae

Many small, round, greyish spots surrounded by a brown border with a more or less distinct yellow halo seen on lower leaves. <b><i>Cercospora nicotianae</i></b> (<i>Cercospora</i> disease, frog-eye)
Numerous small round spots of pale beige on a Virginia tobacco leaf. <i>Cercospora nicotianae</i> (cercospora disease, frog-eye)
In the center of necrotic spots, there are tiny black clusters composed of conidiophores of the fungus bearing characteristic conidia. <b><i>Cercospora nicotianae</i></b> (<i>Cercospora</i> disease, frog-eye)
From the black clusters collected on the leaves, elongated conidia of the fungus are observed. <b><i>Cercospora nicotianae</i></b> (<i>Cercospora</i> disease, frog-eye)